| 质粒名称: | pT3-EF1aH-PIK3CA H1047R |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 180031 |
| 存储实验室: | Xin Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pT3-EF1aH |
| 载体出品公司: | Clontech |
| 空载体大小: | 7000bp |
| 完整质粒大小: | 8867 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PI3K、p110 alpha、PIK3CA H1047R、phosphoinositide 3-kinase |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3207bp |
| 基因突变: | TH1047R |
| GenBank编号 | NM_006218.4 |
| 启动子: | EF1a |
| 标签/融合蛋白: | HA tag (N terminal on insert) |
| 克隆方法: | Gateway Cloning |
| 5’测序引物 | EF1a-F |
|
文献引用方法 |
|
| 材料和方法部分: | pT3-EF1aH-PIK3CA H1047R was a gift from Xin Chen (Addgene plasmid # 180031 ; http://n2t.net/addgene:180031 ; RRID:Addgene_180031) |
| 参考文献部分: | Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to induce liver tumor formation in mice via AKT2/mTORC1 cascade. Wang C, Che L, Hu J, Zhang S, Jiang L, Latte G, Demartis MI, Tao J, Gui B, Pilo MG, Ribback S, Dombrowski F, Evert M, Calvisi DF, Chen X. Liver Int. 2015 Dec 30. doi: 10.1111/liv.13055. 10.1111/liv.13055 PubMed 26716908 |
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pT3-EF1aH-PIK3CA H1047R载体质粒基本信息
pT3-EF1aH-PIK3CA H1047R质粒图谱载体图谱和 pT3-EF1aH-PIK3CA H1047R载体序列质粒序列多克隆位点信息
pT3-EF1aH-PIK3CA H1047R质粒载体简介
pT3-EF1aH-PIK3CA H1047R质粒序列载体序列
LOCUS pT3-EF1aH-PIK3CA H1047R 8867 bp DNA circular SYN 14-APR-2025
DEFINITION This plasmid is in the pT3-EF1a vector without loxP sites flanking
the inverted repeats of SB (sleeping beauty) sequence. Therefore
this plasmid can be used in LoxP mice..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8867)
AUTHORS Wang C, Che L, Hu J, Zhang S, Jiang L, Latte G, Demartis MI, Tao J,
Gui B, Pilo MG, Ribback S, Dombrowski F, Evert M, Calvisi DF, Chen X
TITLE Activated mutant forms of PIK3CA cooperate with RasV12 or c-Met to
induce liver tumor formation in mice via AKT2/mTORC1 cascade.
JOURNAL Liver Int. 2015 Dec 30. doi: 10.1111/liv.13055.
PUBMED 26716908
REFERENCE 2 (bases 1 to 8867)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Liver Int.
2015 Dec 30. doi: 10.1111/liv.13055."
COMMENT Sequence Label: pT3-EF1aH-PIK3CA H1047R
FEATURES Location/Qualifiers
source 1..8867
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 132..151
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 385..402
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 519..540
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(555..560,561..578,579..585)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 593..609
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 598..620
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 617..633
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 617..633
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1059..1078)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
protein_bind 1131..1164
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
promoter 1305..2483
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 1536..2474
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 2431..2451
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
primer_bind 2500..2519
/label=T7
/note="T7 promoter, forward primer"
promoter 2500..2518
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 2597..2621
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 2668..2694
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
protein_bind complement(5937..5961)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 6014..6055
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
primer_bind complement(6093..6110)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6099..6323
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind 6393..6426
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
primer_bind 6482..6502
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
primer_bind complement(6884..6901)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6884..6900)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6893..6915)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(7109..7128)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7228..7250
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7288..7306)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7374..7478
/gene="bla"
/label=AmpR promoter
CDS join(7479..7547,7548..8339)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7697..7716)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin join(8510..8867,1..231)
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
601 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg
661 caggtcgact ctagacctat acagttgaag tcggaagttt acatacactt aagttggagt
721 cattaaaact cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc
781 aagtcagtta ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac
841 agacagatta tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca
901 tacactaagt tgactgtgcc tttaaacagc ttggaaaatt ccagaaaatg atgtcatggc
961 tttagaagct tctgatagac taattgacat catttgagtc aattggaggt gtacctgtgg
1021 atgtatttca aggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
1081 ctagaatgca gcggccgcta gctcgagatc ttaattaaac tagtatcgat ataacttcgt
1141 ataatgtatg ctatacgaag ttatatgtac gggccagata tacgcgttga cattgattat
1201 tgactaggct tttgcaaaaa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
1261 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
1321 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
1381 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
1441 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
1501 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
1561 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
1621 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
1681 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
1741 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
1801 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
1861 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
1921 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
1981 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
2041 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
2101 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
2161 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
2221 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
2281 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
2341 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
2401 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
2461 ttcttccatt tcaggtgtcg tgaggaatta gcttggtact aatacgactc actataggga
2521 gacccaagct ggctaggtaa gcttggtacc gagctcggat ccactagtcc agtgtggtgg
2581 aattctgcag atatcaacaa gtttgtacaa aaaagcaggc tttaaaggaa ccaattcagt
2641 cgactggatc caagcttcac catggggtac ccatacgacg tgccagatta cgccagatcc
2701 cagtgtggtg gtacgtaccc tccaagacca tcatcaggtg aactgtgggg catccacttg
2761 atgcccccaa gaatcctagt agaatgttta ctaccaaatg gaatgatagt gactttagaa
2821 tgcctccgtg aggctacatt aataaccata aagcatgaac tatttaaaga agcaagaaaa
2881 taccccctcc atcaacttct tcaagatgaa tcttcttaca ttttcgtaag tgttactcaa
2941 gaagcagaaa gggaagaatt ttttgatgaa acaagacgac tttgtgacct tcggcttttt
3001 caaccctttt taaaagtaat tgaaccagta ggcaaccgtg aagaaaagat cctcaatcga
3061 gaaattggtt ttgctatcgg catgccagtg tgtgaatttg atatggttaa agatccagaa
3121 gtacaggact tccgaagaaa tgttctgaac gtttgtaaag aagctgtgga tcttagggac
3181 ctcaattcac ctcatagtag agcaatgtat gtctatcctc caaatgtaga atcttcacca
3241 gaattgccaa agcacatata taataaatta gataaagggc aaataatagt ggtgatctgg
3301 gtaatagttt ctccaaataa tgacaagcag aagtatactc tgaaaatcaa ccatgactgt
3361 gtaccagaac aagtaattgc tgaagcaatc aggaaaaaaa ctcgaagtat gttgctatcc
3421 tctgaacaac taaaactctg tgttttagaa tatcagggca agtatatttt aaaagtgtgt
3481 ggatgtgatg aatacttcct agaaaaatat cctctgagtc agtataagta tataagaagc
3541 tgtataatgc ttgggaggat gcccaatttg atgttgatgg ctaaagaaag cctttattct
3601 caactgccaa tggactgttt tacaatgcca tcttattcca gacgcatttc cacagctaca
3661 ccatatatga atggagaaac atctacaaaa tccctttggg ttataaatag tgcactcaga
3721 ataaaaattc tttgtgcaac ctacgtgaat gtaaatattc gagacattga taagatctat
3781 gttcgaacag gtatctacca tggaggagaa cccttatgtg acaatgtgaa cactcaaaga
3841 gtaccttgtt ccaatcccag gtggaatgaa tggctgaatt atgatatata cattcctgat
3901 cttcctcgtg ctgctcgact ttgcctttcc atttgctctg ttaaaggccg aaagggtgct
3961 aaagaggaac actgtccatt ggcatgggga aatataaact tgtttgatta cacagacact
4021 ctagtatctg gaaaaatggc tttgaatctt tggccagtac ctcatggatt agaagatttg
4081 ctgaacccta ttggtgttac tggatcaaat ccaaataaag aaactccatg cttagagttg
4141 gagtttgact ggttcagcag tgtggtaaag ttcccagata tgtcagtgat tgaagagcat
4201 gccaattggt ctgtatcccg agaagcagga tttagctatt cccacgcagg actgagtaac
4261 agactagcta gagacaatga attaagggaa aatgacaaag aacagctcaa agcaatttct
4321 acacgagatc ctctctctga aatcactgag caggagaaag attttctatg gagtcacaga
4381 cactattgtg taactatccc cgaaattcta cccaaattgc ttctgtctgt taaatggaat
4441 tctagagatg aagtagccca gatgtattgc ttggtaaaag attggcctcc aatcaaacct
4501 gaacaggcta tggaacttct ggactgtaat tacccagatc ctatggttcg aggttttgct
4561 gttcggtgct tggaaaaata tttaacagat gacaaacttt ctcagtattt aattcagcta
4621 gtacaggtcc taaaatatga acaatatttg gataacttgc ttgtgagatt tttactgaag
4681 aaagcattga ctaatcaaag gattgggcac tttttctttt ggcatttaaa atctgagatg
4741 cacaataaaa cagttagcca gaggtttggc ctgcttttgg agtcctattg tcgtgcatgt
4801 gggatgtatt tgaagcacct gaataggcaa gtcgaggcaa tggaaaagct cattaactta
4861 actgacattc tcaaacagga gaagaaggat gaaacacaaa aggtacagat gaagttttta
4921 gttgagcaaa tgaggcgacc agatttcatg gatgctctac agggctttct gtctcctcta
4981 aaccctgctc atcaactagg aaacctcagg cttgaagagt gtcgaattat gtcctctgca
5041 aaaaggccac tgtggttgaa ttgggagaac ccagacatca tgtcagagtt actgtttcag
5101 aacaatgaga tcatctttaa aaatggggat gatttacggc aagatatgct aacacttcaa
5161 attattcgta ttatggaaaa tatctggcaa aatcaaggtc ttgatcttcg aatgttacct
5221 tatggttgtc tgtcaatcgg tgactgtgtg ggacttattg aggtggtgcg aaattctcac
5281 actattatgc aaattcagtg caaaggcggc ttgaaaggtg cactgcagtt caacagccac
5341 acactacatc agtggctcaa agacaagaac aaaggagaaa tatatgatgc agccattgac
5401 ctgtttacac gttcatgtgc tggatactgt gtagctacct tcattttggg aattggagat
5461 cgtcacaata gtaacatcat ggtgaaagac gatggacaac tgtttcatat agattttgga
5521 cactttttgg atcacaagaa gaaaaaattt ggttataaac gagaacgtgt gccatttgtt
5581 ttgacacagg atttcttaat agtgattagt aaaggagccc aagaatgcac aaagacaaga
5641 gaatttgaga ggtttcagga gatgtgttac aaggcttatc tagctattcg acagcatgcc
5701 aatctcttca taaatctttt ctcaatgatg cttggctctg gaatgccaga actacaatct
5761 tttgatgaca ttgcatacat tcgaaagacc ctagccttag ataaaactga gcaagaggct
5821 ttggagtatt tcatgaaaca aatgaatgat gcacgtcatg gtggctggac aacaaaaatg
5881 gattggatct tccacacaat taaacagcat gcattgaact gagtcgagat atctagaccc
5941 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg
6001 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
6061 cggttagtaa tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
6121 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
6181 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
6241 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
6301 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
6361 gtatccccac gcgccctgta gcggcgcatt aaataacttc gtataatgta tgctatacga
6421 agttatatcg tatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
6481 caccctaact gacacacatt ccacagaatt ccttgaaata catccacagg tacacctcca
6541 attgactcaa atgatgtcaa ttagtctatc agaagcttct aaagccatga catcattttc
6601 tggaattttc caagctgttt aaaggcacag tcaacttagt gtatgtaaac ttctgaccca
6661 ctggaattgt gatacagtga attataagtg aaataatctg tctgtaaaca attgttggaa
6721 aaatgacttg tgtcatgcac aaagtagatg tcctaactga cttgccaaaa ctattgtttg
6781 ttaacaagaa atttgtggag tagttgaaaa acgagtttta atgactccaa cttaagtgta
6841 tgtaaacttc cgacttcaac tgtagggtac cgagctcgaa ttcactggcc gtcgttttac
6901 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
6961 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
7021 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
7081 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
7141 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
7201 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
7261 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
7321 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
7381 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
7441 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
7501 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
7561 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
7621 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
7681 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
7741 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
7801 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
7861 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
7921 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
7981 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
8041 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
8101 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
8161 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
8221 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
8281 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
8341 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
8401 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
8461 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
8521 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
8581 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
8641 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
8701 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
8761 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
8821 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacg
//
